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Testosterone, Total

Analyte: Testosterone, Total

Specimen Type: Serum, Inquire for additional option(s)

Optimum Volume: 0.5 mL

Stability:

2-8°C -20°C -70°C
6 days 6 months 3 years

Reporting units: ng/mL

Method: Electrochemiluminescence

Biological or Clinical Significance:

The androgen testosterone (17β-hydroxyadrostenone) has a molecular weight of 288 daltons. In men, testosterone is synthesized almost exclusively by the leydig cells of the testes. The secretion of testosterone is regulated by luteinizing hormone (LH), and is subject to negative feedback via the pituitary and hypothalamus. Testosterone promotes the development of the secondary sex characteristics in men and serves to maintain the function of the prostate and seminal vesicles. Most of the circulating testosterone is bound to carrier proteins (SHBG-sex hormone binding globulin). In women, small quantities of testosterone are formed in the ovaries. In physiological concentrations, androgens have no specific effects in women. Increased production of testosterone in women can cause virilization (depending on the increase).

The determination of testosterone in women is helpful in the diagnosis of androgenic syndrome, polycystic ovaries and when an ovarian tumor, adrenal tumor, adrenal hyperplasia, or ovarian insufficiency is suspected. In men, testosterone levels are determined when reduced testosterone production is suspected, e.g. in hypogonadism, estrogen therapy, chromosome aberrations (as in Kleinfelter’s syndrome), and liver cirrhosis.

Principle of Test Method:

The testosterone assay is a competitive immunoassay using electrochemiluminescent detection.

References:

1. Gronowski AM, Landau-Levine M. Reproductive endocrine function. In: Tietz Textbook of Clinical Chemistry, 3rd edition; Burtis CA, Ashwood ER, eds. WB Saunders, Philadelphia, 1999, pp 1601-1639.
2. Vankrieken L. Testosterone and the free androgen index. Published by Diagnostic Products Corporations, May, 1997, pp 1-8.
3. Taieb J, Mathian B, Millot F, Patricot M-C, Mathieu E, Queyrel N, Lacroix I, Somma-Delpero C, Boudou P. Testosterone Measured by 10 immunoassays and by isotope-dilution gas chromatography-mass spectrometry in sera from 116 men, women, and children. Clin Chem. 2003; 49:1381-1395.
4. Wang, D, Catlin DH, Demers LM, Starcevic B, Swerdloff RS. Measurement of total serum testosterone in adult men: Comparison of current Laboratory methods versus liquid chromatography-tandem mass spectrometry. J Clin Endocrinol Metab. 2005; 89:534-543.
5. Bolelli G, Muti P, Micheli A, Sciajno R, Franceschetti F, Krogh V, Pisani P, Berrino F. Validity for epidemiological studies of long-term cryoconservation of steroid and protein hormones in serum and plasma. Cancer Epidemiol Biomarker Prev. 1995; 4:509-513.

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