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Triglycerides, Total (w/o Glycerol Correction)

Analyte: Triglycerides Total

Specimen Type: Serum, Inquire for additional option(s)

Optimum Volume: 0.5 mL

Stability:

2-8°C -20°C -70°C
5 days 3 months 2 years

Reporting units: mg/dL

Method: Enzymatic

Biological or Clinical Significance:

Triglycerides are the major form of fat found in nature, and their primary function is to provide energy for the cells.  Because of their water insolubility, triglycerides are transported in the plasma in combination with other more polar lipids (phospholipids) and proteins, as well as with cholesterol and cholesterol esters, in the complex lipoprotein macromolecules. Determination of triglyceride (TG) on a mass basis assumes that the molecular weight of triolein, 885 g/mol, represents the average triacylglycerol molecule in the circulation.  Therefore, normal levels of glycerol translate into addition of 5 to10 mg of TG per deciliter of serum or plasma.  In normolipidemic subjects, this difference is relatively insignificant, but at higher TG levels the effect is more substantial and should be accounted for.  The levels of TG (and glycerol) are higher in patients with metabolic syndrome and diabetes, as well as a host of genetic conditions or in carriers of some alleles.   In lipid loading studies and other studies investigating triglycerides in postprandial serum or plasma, TG levels are much higher than when fasting. 

Principle of Test Method:

The triglycerides assay is based on the enzymatic determination of glycerol with glycerol phosphase-oxidase (GPO) after hydrolysis with lipoprotein labase. The automated procedure quantitates the total glycerides in serum including the mono and diglycerides and free glycerol fraction.

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