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Fructosamine

Analyte: Fructosamine

Specimen Type: Serum, EDTA Plasma, Heparin Plasma

Optimum Volume: 1 mL

Stability:

2-8°C -20°C -70°C
2 weeks 2 months 6.5 years

Reporting units: umol/L

Method: Enzymatic

Biological or Clinical Significance:

Glucose reversibly reacts with primary amino groups (e.g., on the side chain of lysine or the N-terminus of proteins) to form a Schiff base (aldimine), which in turn slowly rearranges (Amardori rearrangement) to a ketoamine. In the case of protein ketoamines, the resulting covalent complex has a biological half-life that is equal to that of the particular protein involved. In plasma, the average half-life of the protein ketoamines approaches that of albumin, ~20 days, because albumin is by far the most abundant plasma protein. The concentration of fructosamine, the ketoamine resulting from glucose amino group reaction, reflects the average concentration of glucose during the weeks before a blood samples is obtained. Repeated measurement of the fructosamine is generally used to monitor changes in glucose status in diabetics during treatment during the 2-3 weeks prior to blood sampling. 

Principle of Test Method: The fructosamine is an automated enzymatic assay.

References:

1. Johnson RN, Metcalf PA, Baker JR. Fructosamine: a new approach to the estimation of serum glycosylprotein. An index of diabetic control. Clin Chim Acta 1983 Jan 7;127:87-95.
2. Tahara Y, Shima K. Kinetics of HbA1c, glycated albumin, and fructosamine and analysis of their weight functions against preceding plasma glucose level. Diabetes Care 1995 Apr;18(4):440-7.

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