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Analyte: Macrophage Inflammatory Protein-3 beta
Specimen Type: Serum
Optimum Volume: 0.4 mL
|6 days||23 days||2 years|
Reporting units: pg/mL
Biological or Clinical Significance:
MIP-3 beta also referred to as CCL19, ELC, Exodus-3, and SCYA19 is a CC chemokine that is expressed in secondary lymphoid organs like the thymus, lymph nodes as well as in activated bone marrow stromal cells. CCL19 signals via the CCR7 receptors and is a strong chemoattractant for T and B lymphocytes but not for monocytes and granulocytes. MIP3 beta plays an important role in lymphocyte trafficking by promoting infiltration of T-cells and dendritic cells into lymphoid tissue. MIP-3 beta also promotes T-cell homing into non-lymphoid tissue and may stimulate inflammatory responses. Thus, CCL19/CCL21/CCR7 has been implicated in the pathogenesis of various autoimmune disorders such as rheumatoid arthritis, systemic lupus erythematosis, diabetes mellitus, inflammatory bowel disease, as well as coronary artery disease. Elevated MIP-3 beta levels have been observed in many of these diseases as well as in HIV and HCV infected patients. Systemic lupus erythematosis may be the most relevant as elevated MIP-3 beta along with elevated IP-10 and MCP-1 levels showed a strong correlation with current disease activity.
Principle of Test Method:
The human MIP-3 beta is a solid-phase ELISA that may be used to measure MIP-3 beta in cell culture supernates, serum, plasma, and urine. This assay employs the quantitative sandwich enzyme immunoassay technique.
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