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VLDL-Apolipoproteins C-II

Analyte: VLDL Apolipoproteins CII

Specimen Type: Serum, EDTA Plasma

Optimum Volume: 2.5 mL*

Stability:

2-8°C -20°C -70°C
1 week 3 months 2.5 years

Reporting units: mg/dL

Method: Ultracentrifugation & Immunoturbidimetric

Biological or Clinical Significance:

Apolipoproteins are components of lipoproteins in plasma and are important in determining the structure, function and metabolism of lipoproteins. Apolipoprotein C-II is contained in very low density lipoproteins (VLDL) and high density lipoproteins (HDL), and activates lipoprotein lipase (LPL) on the surface of endothelial cells. In type II dyslipidemia patients a strong positive correlation between plasma TG and apo C-II and C-III concentrations exists. However, the most pronounced increase in plasma apolipoproteins is observed in type V dyslipidemia patients. In these patients, plasma TG are positively correlated with apo C-III and inversely correlated with apo C-II.

Principle of Test Method:

In this method, the bottom fraction is obtained by ultracentrifugation (see LDL-C by ultracentrifugation). Apolipoprotein CII is then measured in both the whole sample and bottom fraction using an immunoturbidimetric method.  The VLDL Apo CII is obtained by calculating the difference between the Apo CII measured in the whole sample and the Apo CII measured in the bottom fraction.     *1.2 mL minimum volume; 2.5 mL allows for a repeat if needed.

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