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Specimen Type: Serum, Inquire for addtional option(s)
Optimum Volume: 0.5 mL
|5 days||11 months||2 years|
Reporting units: pg/mL
Method: ECLIA (Electrochemiluminescent Immunoassay)
Biological or Clinical Significance:
Interleukin-10 (IL-10) is a potent modulator of monocyte/macrophage function. Human IL-10 cDNAs encode 178 amino acid (aa) residue precursor polypeptides with hydrophobic signal peptides that are cleaved to generate 160 aa mature proteins that appear to be non-glycosylated. As a down-regulator of the cell-mediated immune response, IL-10 can suppress the production of prostaglandin E2 and numerous pro-inflammatory cytokines, including TNF-α, IL-1, IL-6, and IL-8 by monocytes following activation. IL-10 also enhances the release of soluble TNF receptors and inhibits the expression of surface ICAM-1 and B7. Finally, IL-10 has been reported to suppress the synthesis of superoxide anion plus reactive oxygen intermediates (ROI), and either inhibits or facilitates NO synthesis, depending on the time of exposure to activated macrophages.
IL-10 also has marked effects on B cells. Aside from its activities on B lymphocytes, IL-10 also has documented activity on endothelial cells. On neutrophils, IL-10 has been reported to diminish secretion of TNF-α, IL-1β, IL-8, MIP-1α and MIP-1β after LPS challenge. Further, IL-10 upregulates IL-1ra expression from LPS-stimulated neutrophils, suggesting a role for IL-10 as an anti-inflammatory molecule.
Principle of Test Method:
The IL-10 assay is a sandwich immunoassay using eletrochemiluminescent detection.
1. Baiocchi OCG, Colleoni GWB, Caballero OL, Vettore AL, Bulgarelli A, Dalbone MA, Granato CFH, Franco MF, Pestana JOM. Epstein-Barr viral load, interleukin-6 and interleukin-10 levels in post-transplant lymphoproliferative disease: A nested case-control study in a renal transplant cohort. Leuk Lymphoma. 2005; 46:533-539.
3. Harizi H, Gualde N. Pivotal role of PGE2 and IL-10 in the cross-regulation of dendritic cell-derived inflammatory mediators. Cell Mol Immunol. 2006; 3:271-277.