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PINP (N-Terminal Propeptide of Type I Collagen) - ECL

Analyte: Aminoterminal Propeptide of Type I Collagen

Specimen Type: Serum, Inquire for additional option(s)

Optimum Volume: 0.5 mL

Stability:

2-8°C -20°C -70°C
3 days 2 years 2 years

Reporting units: ng/mL

Method: Electrochemiluminescence

Biological or Clinical Significance:

Collagen type I is present in soft connective tissues and bone, where it constitutes more than 90% of the organic matrix. During bone formation collagen type I is synthesized from pro-collagen type I, which is secreted from fibroblasts and osteoblasts. Pro-collagen type I contains N-and C-terminal extensions, which are removed by specific proteases during the conversion of procollagen to collagen. The extensions are referred to as the C- and N- terminal propeptides of procollagen type I (PICP and PINP). Thus, measurements of PINP can be of value in assessing bone formation.

Principle of Test Method:

The PINP assay is a sandwich immunoassay using electrochemiluminescent detection.

References:

1. Brandt J, Krogh TN, Jensen CH, et al., Thermal Instability of the Trimeric Structure of the N-terminal Propeptide of Human Procollagen Type I in Relation to Assay Technology. Clin Chem 1999, 45: 47-53.
2. Roche Diagnostics, A First Technical & Clinical Review of a New Automated serum P1NP Assay. Presented at: 11th International Congress of Endocrinology, Sydney, Nov. 2000, paper number P0331.
3. Melkko J, Kauppila S, Niemi S, et al., Immunoassay for intact amino-terminal propeptide of human type I procollagen. Clin Chem 1996, 42: 947-954.
4. Leary E, Carlson T, Wu C, Aggoune T, Luzzi V, Foster AP. Serum CTX and P1NP by Roche Elecsys as teranostics in the treatment of osteoporosis. JBMR 2005, 20:S227.
5. Garnero, P., Vergnaud, P., Hoyle, N., “Evaluation of Fully Automated Serum Assay for Total N-Terminal Propeptide of Type I Collagen in Postmenopausal Osteoporosis” Clin Chem. 2008; 54:1 pg 188-196.

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